rabbit anti psd95  (Alomone Labs)

Journal: Frontiers in Endocrinology

Article Title: Dorzagliatin shows potential in preventing cognitive impairment in diabetes: evidence from Mendelian randomization analysis and animal study

doi: 10.3389/fendo.2025.1755359

Figure Lengend Snippet: Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.

Article Snippet: The membranes were blocked with 1×TBST and 5% BSA (tank blotting) or 5% skim milk (Semi-dry blotting) for 1 hour at room temperature and then incubated overnight at 4°C with following primary antibodies, respectively: mouse monoclonal anti-GluN1 antibody (Millipore, Cat. No: 05-432); rabbit polyclonal anti-GluN2A antibody (NOVUS, Cat. No: NB300-105); rabbit monoclonal anti-GLUT1 antibody (Abcam, Cat. No: ab115730); rabbit polyclonal anti-GLUT3 antibody (Bioss, Cat. No: bs-1207R); rabbit polyclonal anti-IR antibody (Abcam, Cat. No: ab137747); rabbit monoclonal anti-PSD95 antibody (Abcam, Cat. No: ab238135).

Techniques: Western Blot, Control, Two Tailed Test

Journal: bioRxiv

Article Title: Disrupted maternal care alters neural-microglia interactions in the primate paralaminar (PL) nucleus of the amygdala

doi: 10.64898/2026.01.06.697965

Figure Lengend Snippet: A. Low-magnification (4x) overview of the paralaminar nucleus (PL) ventral to the basal amygdala, highlighting the location of the analysis identified by (DCX+) neurons. Scale bar: 300 µm. B. High-magnification (60x) photomicrograph of the PL neuropil depicting SYN1(green) and PSD95 (red) immunoreactivity. Scale bar: 10 µm. C. Spot rendering of SYN1 (green) and PSD95 (red) puncta at low power. Scale bar: 10 μm. D. Putative synaptic contacts (SYN1 and PSD95 overlap, greater than 50%) in yellow, double arrow. Single arrows show SYN1 and PSD95 puncta pairs that are non-overlapping. Scale bar = 2 μm. E. Mean number of SYN1-single labeled puncta across groups. F. mean number of PSD95-single labeled puncta across groups. G. Mean number of putative synaptic contacts (SYN1-PSD95 colocalized) across groups. H. Percentage of SYN1 contacts per total available SYN1+ puncta. I. Percentage of PSD95 contacts per total available PSD95+ puncta. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Based on these measurements, we applied a 1 μm maximum XY spot diameter for SYN1 and PSD95 puncta in Imaris.

Techniques: Labeling

Journal: bioRxiv

Article Title: Disrupted maternal care alters neural-microglia interactions in the primate paralaminar (PL) nucleus of the amygdala

doi: 10.64898/2026.01.06.697965

Figure Lengend Snippet: A-C . Representative 3D spot-surface renderings for pre-synaptic elements (green)( A.) post-synaptic excitatory elements (pink ) (B), and putative contacts (SYN1-PSD95 colocalized, yellow) (C). D-F. Individual microglia isolation method, 10 microglia/animal. D. Mean number of engulfed presynaptic (SYN1+) elements per microglia. E. Mean number of engulfed postsynaptic (PSD95+) elements per microglia. F. Mean number of engulfed putative synaptic contacts (SYN1-PSD95 overlap) per microglia. G-I. Quantitative results from the ROI neuropil analyses, 10 (3x10 4 µm 3 ) ROIs/animal. G. Mean number of engulfed presynaptic (SYN1+) elements per neuropil ROI H. Mean number of engulfed postsynaptic (PSD95+) elements per neuropil ROI. I. Mean number of engulfed putative synaptic contacts (SYN1-PSD95 overlap) per neuropil ROI. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Based on these measurements, we applied a 1 μm maximum XY spot diameter for SYN1 and PSD95 puncta in Imaris.

Techniques: Isolation